Tez No |
İndirme |
Tez Künye |
Durumu |
453624
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Determination of genetic diversity of rhododendron species in turkey / Türkiye orman güllerinin genetik çeşitliliğinin belirlenmesi
Yazar:İSMAİL TUĞBERK KAYA
Danışman: PROF. DR. MÜGE TÜRET
Yer Bilgisi: Boğaziçi Üniversitesi / Fen Bilimleri Enstitüsü / Moleküler Biyoloji ve Genetik Ana Bilim Dalı / Moleküler Biyoloji Bilim Dalı
Konu:Biyoloji = Biology ; Genetik = Genetics
Dizin:Biyoloji = Biology ; Genetik varyasyon = Genetic variation ; Polimorfizm-genetik = Polymorphism-genetic
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Onaylandı
Yüksek Lisans
İngilizce
2017
148 s.
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Turkiye'nin Ormangulleri 10 takson ile temsil edilmekte ve dogudaki Artvin ilinden
batdaki Istranca daglarna kadar Karadeniz ve Marmara kylarnda dogal olarak
buyumektedirler. Gunumuzde Rhododendron cinsi icerisinde yer alan bitkiler, karakteristik
ozelliklerinden dolay ds mek^an duzenlemelerinde ve saksl sus bitkisi olarak
kullanm acsndan son derece populer bitkilerdir. Kentsel peyzaj planlamalarnda
kulture alnms dogal orman gulu turlerinin yan sra slah calsmalar ile gelistirilmis ve
gunumuzde saylar binlerle ifade edilen orman gulu cesitleri de skca kullanlmaktadr.
Ulkemizde dogal olarak yayls gosteren orman gulleri gosterisli sus bitkileri olmalarna
ragmen yerli turler henuz kulture alnmadg icin kentsel peyzaj uygulamalarnda sus
bitkisi olarak kullanlmamaktadr. Peyzaj planlamalarda kullanlan orman gulleri ise
ithal edilerek, yuksek yatlarla tuketiciye sunulmaktadr. Turkiye'nin dogal olarak
yetisen Rhododendron turlerinin ex-situ korunmas icin iyi belgelendirilmis bir kaynak
olusturmak amacyla ulusal bir proje baslatlmstr. Bu projenin ortaklar olarak, yer
ve yuksekligin cesitlilige olas etkilerini de dahil ederek genetik cesitliligi analiz etmeyi
amaclanmstr. Ornekler arasndaki genetik cesitlilik ve logenetik iliskiyi incelemek
icin RAPD, ISSR ve ITS isaretcilerinden olusan toplam 22 primer secilmistir. Kume
analizi olusturmak icin veri matrisinin genetik uzaklk hesaplamalar, Pearson ve Jaccard
katsays kullanlarak UPGMA metodu ile gerceklestirilmistir. Genetik benzerligi
yanstan dendogramlar MATLAB kullanlarak cizilmistir. Tum primerler, 200-3000 bp
arasnda degisen amplikon uretmis ve % 100 polimorzm gostermistir. Cogra aralgn
tur genetik cesitliligi uzerindeki etkisi, oldukca genis yaylms yabani turlerin cok dar
dogal gelisim yerlerine sahip diger yabani turlerden ayr gruplar halinde kumelenmesyle
ackca gorulmustur.
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The Rhododendrons of Turkey are presented by 10 taxa and they grow naturally
along the coast of the Black Sea region from Artvin province at the east to
Istranca Mountains at the west. Although in many countries, cultivated natural forms
or breeding varieties of Rhododendron genus are highly used in landscape planning and
as ornamentals, in Turkey there are no cultivated local forms available and all commercial
varieties are imported as potted plants. Recently, a national project has started
with an objective to generate well documented ex-situ conservation of all naturally
growing Rhododendron species of Turkey following a detailed morphological, molecular
studies and tissue culture propagations. As partners of this project our aim is to
analyze the genetic diversity by incorporating possible impacts of location and altitude
to their diversity. The plant material has been collected from nature at dierent GPS
locations and diverse altitudes along the cost of Black Sea and Marmara region. Total
of 22 primers consisting of RAPD, ISSR and ITS markers were selected to study the
genetic diversity and phylogenetic relationship among the samples. Genetic distance
calculations of the data matrix to generate cluster analysis were performed by UPGMA
using Pearson and Jaccard coecient. The dendograms refecting the genetic distance
were drawn using MATLAB. All primers have shown 100% polymorphism, producing
amplicons ranging between 200-3000bp. The average number of bands obtained for
ISSR and RAPD primers were 19.1 and 20.9 respectively. The impact of geographic
range on the species genetic diversity were clearly observed in cluster analysis when
highly wide spread wild species were clustered as separate groups from the other wild
species which has very narrow range of natural growth locations. |