Tez No	İndirme	Tez Künye	Durumu
183943		Eritrositlerde L-sistein amino asit exportuna hücre içi ve dışı redoks durumunun etkisi / The effect of intracellular and extracellular redox status on L-cysteine export in erythrocytes Yazar:CEYLAN USLU Danışman: DOÇ.DR. DENİZ YILDIZ Yer Bilgisi: Mustafa Kemal Üniversitesi / Fen Bilimleri Enstitüsü / Genel Biyoloji Ana Bilim Dalı Konu:Biyokimya = Biochemistry ; Biyoloji = Biology Dizin:	Onaylandı Yüksek Lisans Türkçe 2006 53 s.
1ÖZET(5ø7526ø7/(5'(/6ø67(ø1$0ø12$6ø7(;325781$+h&5(ødø9(',ù,5('2.6'85808181(7.ø6øGlutatyon, antioksidan savunmada önemli bir rol oynar. Bu nedenle glutatyon,eritrositlerde yüksek miktarlarda bulunur. L-sistein amino asidi, eritrositlerde glutatyonVHQWH]L LoLQ JHUHNPHNWHGLU %X oDOÕúPDQÕQ DPDFÕ KFUH LoL YH GÕúÕ redoks durumunun,eritrositlerde L-sistein HNVSRUWXQD HWNLVLQL LQFHOHPHNWLU dDOÕúPDGD HULWURVLWOHUL IDUNOÕkonsantrasyonlardaki L-sistein DPLQR DVLGLQH PDUX] EÕUDNÕOPÕú YH VRQUD GDHULWURVLWOHUGHNL VHUEHVW ±6+ NRQVDQWUDV\RQXQX |OoOPúWU $\QÕ \|QWHPOH PXDPHOHedilen eritrositler, sonraki L-VLVWHLQ oÕNÕúÕ oDOÕúPDODUÕQGD NXOODQÕOPÕúWÕU $\QÕ ]DPDQGDÕVÕQÕQ EX JLULúoÕNÕúD RODQ HWNLVL GH÷HUOHQGLULOPLúWLU øON RODUDN ELU glutatyon tüketicisiolan 1-chloro-2,4-dinitrobenzene ile muamele edilen eritrositlerde, buthionineVXOIR[LPLQHYDUOÕ÷ÕQGDYH\RNOX÷XQGD/VLVWHLQoÕNÕúÕQÕQQDVÕOHWNLOHQGL÷LEHOLUOHQPLúWLUL-VLVWHLQ JLULúL oDOÕúPDODUÕPÕ]GD SOD]PDGD /VLVWHLQ NRQVDQWUDV\RQX DUWWÕ÷ÕQGDHULWURVLWOHULQ EX DUWÕúD FHYDS YHUHUHN /sistein DPLQR DVLGLQL KFUH LoLQH DOGÕ÷ÕJ|VWHULOPLúWLU (ULWURVLWOHUGHNL VHUEHVW ±6+ NRQVDQWUDV\RQX P0 /sisteinX\JXODQGÕ÷ÕQGDVDDWWH“P0¶D \NVHOLUNHQEXNRQVDQWUDV\RQ P0 /VLVWHLQ X\JXODQGÕ÷ÕQGD “ P0¶ D \NVHOPLúWLU (ULWURVLWOHUGH VHUEHVW ±6+NRQVDQWUDV\RQODUÕ P0 /VLVWHLQ X\JXODQGÕ÷ÕQGD GDNLNDGD “ P0¶ D\NVHOLUNHQVDDWWHEXNRQVDQWUDV\RQ“P0¶D\NVHOPLúWLU$\QÕ]DPDQGDL-sistein DPLQR DVLGLQLQ oÕNÕúÕQÕQ GD ]DPDQ YH NRQVDQWUDV\RQD ED÷OÕ ROGX÷XEHOLUOHQPLúWLU <NVHN PLNWDUGD /VLVWHLQOH PXDPHOH HGLOPLú HULWURVLWOHUGH \NVHNmiktarda L-VLVWHLQoÕNÕúÕJ|UOUP0/sisteinle muamele edilen eritrositlerde, hücreGÕúÕVHUEHVW±6+NRQVDQWUDV\RQXVDDWWH“P0¶D\NVHOLUNHQP0/sisteinle muamele edilen eritrositlerde bu konsantrasyon 1,014 ± 0,002 mM' a\NVHOPLúWLU ƒ& VÕFDNOÕN HULWURVLWOHUH /VLVWHLQ JLULúLQL WHVW HGLOHQ EWQkonsantrasyonlarda, önemli derecede LQKLEH HWPLúWLU 6ÕFDNOÕN DUWÕúÕ EX JLULú ROD\ÕQÕ\HQLGHQ G]HOWPHNWHGLU %HQ]HU VRQXoODU oÕNÕú oDOÕúPDODUÕ LOH GH HOGH HGLOPLúWLU6RQXoODUÕPÕ] D\QÕ ]DPDQGD /VLVWHLQLQ LoHUL YH GÕúDUÕ transportunun, eritrositinRNVLGDWLI GXUXPXQGDQ HWNLOHQGL÷LQL J|VWHUPLúWLU *OXWDW\RQ WNHWLOGL÷LQGH YH glutatyonVHQWH]L HQJHOOHQGL÷LQGH /VLVWHLQLQ KFUH\H JLULúoÕNÕúÕ |QHPOL GHUHFHGH D]DOPÕúWÕUEritrositler, plazma UHGRNV GXUXPXQXQ GHYDPÕ YH KFUH LoL JOXWDW\RQXQ EHOLUOHGL÷L /VLVWHLQoÕNÕúRUDQÕQGDUROR\QDU2006, 46 sayfaAnahtar Kelimeler: Eritrosit, 6LVWHLQoÕNÕúÕRedoks dengesi.
ABSTRACTTHE EFFECT OF INTRACELLULAR AND EXTRACELLULAR REDOX67$78621/&<67(ø1((;3257,1(5<7+52&<7(6Glutathione has an important role in antioxidant defense. For this reason,glutathione is found at rather high amounts in erythrocytes. L-cysteine is required forglutathione synthesis in erythrocytes. The objective of this study was to invastigate theeffect of intracellular and extracellular redoks status on L-cysteine exports inerythrocytes. In the present study, we exposed the erythrocytes to differentconcentrations of L-cysteine and then measured the intracellular free -SHconcentrations. The erythrocytes treated in the same manner were later utilized for thecysteine efflux studies. The effect of temperature on the influx and the efflux processeswere also evaluated. We also determined the rate of L-cysteine efflux in the presenceand absence of Buthionine Sulfoximine in erythrocytes that are pretreated with 1-chloro-2, 4-dinitrobenzene, a glutathione depleter.Our L-cysteine influx studies demonstrated that erythrocytes can respond toincreases in L-cysteine concentration in the extracellular media and influx L-cysteine ina concentration-dependent manner. Free -SH concentration in erythrocytes treated with1 mM L-cysteine reached to 1,64 ± 0,06 mM in 1 hour whereas this concentrationreached to 4,30 ± 0,01 mM in 10 mM L-cysteine treated erythrocytes. Free -SHconcentration in erythrocytes treated with 10 mM L-cysteine reach to 2,48 ± 0,09 mMin 10 minute whereas this concentration, when treated 10 mM L-cysteine in 1 hour,reach to 4,30 ± 0,01 mM. The L-cysteine efflux is also determined to be time andconcentration-dependent. Erythrocytes that are pretreated with higher L-cysteineconcentrations displayed a higher efflux process. Outside concentration of free -SH in 1mM L-cysteine pretreated erythrocytes, reach to 0,200 ± 0,005 mM in 1hour, whereasthis concentration reach to 1,014 ± 0,002 mM with 10 mM L-cysteine pretreatederythrocytes. The influx process is significantly inhibited +4 ºC at all of theconcentration tested. Increasing of the temperature later recovered the influx process.Similar results were obtained with the efflux studies. Our results also indicate that rateof inward and outward transport of L-cysteine is affected by the oxidative status oferythrocytes. When glutathione is depleted and glutathione synthesis is blocked, L-cysteine uptake and the efflux processes are significantly decreased. Erythrocytes play arole in regulation the plasma redoks status and intracellular level of glutathionedetermines the rate of the L-cysteine efflux.2006, 46 PagesKeywords: Erythrocytes, Cysteine efflux, Redox homeostasis